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Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome
Current technologies used to generate CRISPR/Cas gene perturbation reagents are labor intense and require multiple ligation and cloning steps. Furthermore, increasing gRNA sequence diversity negatively affects gRNA distribution, leading to libraries of heterogeneous quality. Here, we present a rapid...
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| Publicat a: | eLife |
|---|---|
| Autors principals: | , , , , , , , , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
eLife Sciences Publications, Ltd
2019
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6424562/ https://ncbi.nlm.nih.gov/pubmed/30838976 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.42549 |
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