Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

Current technologies used to generate CRISPR/Cas gene perturbation reagents are labor intense and require multiple ligation and cloning steps. Furthermore, increasing gRNA sequence diversity negatively affects gRNA distribution, leading to libraries of heterogeneous quality. Here, we present a rapid...

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Dades bibliogràfiques
Publicat a:eLife
Autors principals: Wegner, Martin, Diehl, Valentina, Bittl, Verena, de Bruyn, Rahel, Wiechmann, Svenja, Matthess, Yves, Hebel, Marie, Hayes, Michael GB, Schaubeck, Simone, Benner, Christopher, Heinz, Sven, Bremm, Anja, Dikic, Ivan, Ernst, Andreas, Kaulich, Manuel
Format: Artigo
Idioma:Inglês
Publicat: eLife Sciences Publications, Ltd 2019
Matèries:
Cell Biology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6424562/
https://ncbi.nlm.nih.gov/pubmed/30838976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.42549
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