Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

Current technologies used to generate CRISPR/Cas gene perturbation reagents are labor intense and require multiple ligation and cloning steps. Furthermore, increasing gRNA sequence diversity negatively affects gRNA distribution, leading to libraries of heterogeneous quality. Here, we present a rapid...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Wegner, Martin, Diehl, Valentina, Bittl, Verena, de Bruyn, Rahel, Wiechmann, Svenja, Matthess, Yves, Hebel, Marie, Hayes, Michael GB, Schaubeck, Simone, Benner, Christopher, Heinz, Sven, Bremm, Anja, Dikic, Ivan, Ernst, Andreas, Kaulich, Manuel
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2019
Assuntos:
Cell Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6424562/
https://ncbi.nlm.nih.gov/pubmed/30838976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.42549
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