Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

Current technologies used to generate CRISPR/Cas gene perturbation reagents are labor intense and require multiple ligation and cloning steps. Furthermore, increasing gRNA sequence diversity negatively affects gRNA distribution, leading to libraries of heterogeneous quality. Here, we present a rapid...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
izdano v:eLife
Main Authors: Wegner, Martin, Diehl, Valentina, Bittl, Verena, de Bruyn, Rahel, Wiechmann, Svenja, Matthess, Yves, Hebel, Marie, Hayes, Michael GB, Schaubeck, Simone, Benner, Christopher, Heinz, Sven, Bremm, Anja, Dikic, Ivan, Ernst, Andreas, Kaulich, Manuel
Format: Artigo
Jezik:Inglês
Izdano: eLife Sciences Publications, Ltd 2019
Teme:
Cell Biology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6424562/
https://ncbi.nlm.nih.gov/pubmed/30838976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.42549
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki