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Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues

Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor microtubule nucleators. Usually, microtubule assemb...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Ku, Siou, Heichette, Claire, Duchesne, Laurence, Chrétien, Denis
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842541/
https://ncbi.nlm.nih.gov/pubmed/33659378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3714
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