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Super-resolution Microscopy-based Bimolecular Fluorescence Complementation to Study Protein Complex Assembly and Co-localization

Numerous experimental approaches exist to study interactions between two subunits of a large macromolecular complex. However, most methods do not provide spatial and temporal information about binding, which are critical for dissecting the mechanism of assembly of nanosized complexes in vivo. While...

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Bibliographic Details
Published in:Bio Protoc
Main Authors: Chen, Jingjing, Yu, Zulin, Unruh, Jay R., Slaughter, Brian D., Jaspersen, Sue L.
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842369/
https://ncbi.nlm.nih.gov/pubmed/33654748
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3524
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