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Live Cell Measurement of the Intracellular pH of Yeast by Flow Cytometry Using a Genetically-Encoded Fluorescent Reporter

The intracellular pH of yeast is a tightly regulated physiological cue that changes in response to growth state and environmental conditions. Fluorescent reporters, which have altered fluorescence in response to local pH changes, can be used to measure intracellular pH. While microscopy is often use...

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Détails bibliographiques
Publié dans:Bio Protoc
Auteurs principaux: Triandafillou, Catherine G., Drummond, D. Allan
Format: Artigo
Langue:Inglês
Publié: Bio-Protocol 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842330/
https://ncbi.nlm.nih.gov/pubmed/33659323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3653
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