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Live Cell Measurement of the Intracellular pH of Yeast by Flow Cytometry Using a Genetically-Encoded Fluorescent Reporter

The intracellular pH of yeast is a tightly regulated physiological cue that changes in response to growth state and environmental conditions. Fluorescent reporters, which have altered fluorescence in response to local pH changes, can be used to measure intracellular pH. While microscopy is often use...

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Detaylı Bibliyografya
Yayımlandı:Bio Protoc
Asıl Yazarlar: Triandafillou, Catherine G., Drummond, D. Allan
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Bio-Protocol 2020
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842330/
https://ncbi.nlm.nih.gov/pubmed/33659323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3653
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