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Live Cell Measurement of the Intracellular pH of Yeast by Flow Cytometry Using a Genetically-Encoded Fluorescent Reporter

The intracellular pH of yeast is a tightly regulated physiological cue that changes in response to growth state and environmental conditions. Fluorescent reporters, which have altered fluorescence in response to local pH changes, can be used to measure intracellular pH. While microscopy is often use...

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Bibliografiska uppgifter
I publikationen:Bio Protoc
Huvudupphovsmän: Triandafillou, Catherine G., Drummond, D. Allan
Materialtyp: Artigo
Språk:Inglês
Publicerad: Bio-Protocol 2020
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842330/
https://ncbi.nlm.nih.gov/pubmed/33659323
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3653
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