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A measure of endosomal pH by flow cytometry in Dictyostelium

BACKGROUND: Dictyostelium amoebae are frequently used to study the organization and function of the endocytic pathway, and specific protocols are essential to measure the dynamics of endocytic compartments and their internal pH. FINDINGS: We have revisited these classical protocols to measure more a...

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Autors principals: Marchetti, Anna, Lelong, Emmanuelle, Cosson, Pierre
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2632630/
https://ncbi.nlm.nih.gov/pubmed/19138423
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-2-7
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