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Cleavable Affinity Purification (Cl-AP): A One-step Procedure to Affinity Purify Protein Complexes
Cleavable Affinity Purification (Cl-AP) uses a tripartite system of Protein-A-Streptavidin beads and nanobodies, coupled with a biotinylated, thiol-cleavable linker, providing one-step affinity purification from lysates of tissues expressing tagged proteins. This technique allows fluorescent version...
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| Udgivet i: | Bio Protoc |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Bio-Protocol
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7842289/ https://ncbi.nlm.nih.gov/pubmed/33659473 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3821 |
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