A Photoclick‐Based High‐Throughput Screening for the Directed Evolution of Decarboxylase OleT

Enzymatic oxidative decarboxylation is an up‐and‐coming reaction yet lacking efficient screening methods for the directed evolution of decarboxylases. Here, we describe a simple photoclick assay for the detection of decarboxylation products and its application in a proof‐of‐principle directed evolut...

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Bibliographische Detailangaben
Veröffentlicht in:Chemistry
Hauptverfasser: Markel, Ulrich, Lanvers, Pia, Sauer, Daniel F., Wittwer, Malte, Dhoke, Gaurao V., Davari, Mehdi D., Schiffels, Johannes, Schwaneberg, Ulrich
Format: Artigo
Sprache:Inglês
Veröffentlicht: John Wiley and Sons Inc. 2020
Schlagworte:
Communications
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7839715/
https://ncbi.nlm.nih.gov/pubmed/32955127
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202003637
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