A Photoclick‐Based High‐Throughput Screening for the Directed Evolution of Decarboxylase OleT

Enzymatic oxidative decarboxylation is an up‐and‐coming reaction yet lacking efficient screening methods for the directed evolution of decarboxylases. Here, we describe a simple photoclick assay for the detection of decarboxylation products and its application in a proof‐of‐principle directed evolut...

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Vydáno v:Chemistry
Hlavní autoři: Markel, Ulrich, Lanvers, Pia, Sauer, Daniel F., Wittwer, Malte, Dhoke, Gaurao V., Davari, Mehdi D., Schiffels, Johannes, Schwaneberg, Ulrich
Médium: Artigo
Jazyk:Inglês
Vydáno: John Wiley and Sons Inc. 2020
Témata:
Communications
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7839715/
https://ncbi.nlm.nih.gov/pubmed/32955127
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202003637
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