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A Photoclick‐Based High‐Throughput Screening for the Directed Evolution of Decarboxylase OleT

Enzymatic oxidative decarboxylation is an up‐and‐coming reaction yet lacking efficient screening methods for the directed evolution of decarboxylases. Here, we describe a simple photoclick assay for the detection of decarboxylation products and its application in a proof‐of‐principle directed evolut...

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Detalhes bibliográficos
Publicado no:Chemistry
Main Authors: Markel, Ulrich, Lanvers, Pia, Sauer, Daniel F., Wittwer, Malte, Dhoke, Gaurao V., Davari, Mehdi D., Schiffels, Johannes, Schwaneberg, Ulrich
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7839715/
https://ncbi.nlm.nih.gov/pubmed/32955127
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202003637
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