Cargando...
SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs
The SARS-CoV-2 3a protein is a putative ion channel implicated in virus life cycle and pathogenesis. We recently expressed, purified, and reconstituted 3a into lipid nanodiscs to solve its structure by cryo-EM to 2.1 Å resolution. In this chapter, we describe methods we developed in order to facilit...
Guardado en:
| Publicado en: | Methods Enzymol |
|---|---|
| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Elsevier Inc.
2021
|
| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7839409/ https://ncbi.nlm.nih.gov/pubmed/34099172 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2020.12.020 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|