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N-terminal protein labeling with N-hydroxysuccinimide esters and microscale thermophoresis measurements of protein-protein interactions using labeled protein

Protein labeling strategies have been explored for decades to study protein structure, function, and regulation. Fluorescent labeling of a protein enables the study of protein-protein interactions through biophysical methods such as microscale thermophoresis (MST). MST measures the directed motion o...

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Detalhes bibliográficos
Publicado no:Curr Protoc
Main Authors: Jiang, Hanjie, Cole, Philip A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7839251/
https://ncbi.nlm.nih.gov/pubmed/33484499
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.14
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