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Sample Preparation and Imaging Conditions Affect mEos3.2 Photophysics in Fission Yeast Cells

Photoconvertible fluorescent proteins (PCFPs) are widely used in super-resolution microscopy and studies of cellular dynamics. However, our understanding of their photophysics is still limited, hampering their quantitative application. For example, we do not know the optimal sample preparation metho...

पूर्ण विवरण

में बचाया:
ग्रंथसूची विवरण
में प्रकाशित:Biophys J
मुख्य लेखकों: Sun, Mengyuan, Hu, Kevin, Bewersdorf, Joerg, Pollard, Thomas D.
स्वरूप: Artigo
भाषा:Inglês
प्रकाशित: The Biophysical Society 2021
विषय:
ऑनलाइन पहुंच:https://ncbi.nlm.nih.gov/pmc/articles/PMC7820738/
https://ncbi.nlm.nih.gov/pubmed/33217381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.11.006
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