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Sample Preparation and Imaging Conditions Affect mEos3.2 Photophysics in Fission Yeast Cells
Photoconvertible fluorescent proteins (PCFPs) are widely used in super-resolution microscopy and studies of cellular dynamics. However, our understanding of their photophysics is still limited, hampering their quantitative application. For example, we do not know the optimal sample preparation metho...
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| Publié dans: | Biophys J |
|---|---|
| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
The Biophysical Society
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7820738/ https://ncbi.nlm.nih.gov/pubmed/33217381 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.11.006 |
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