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Rapid and real-time identification of fungi up to species level with long amplicon nanopore sequencing from clinical samples

The availability of long-read technologies, like Oxford Nanopore Technologies, provides the opportunity to sequence longer fragments of the fungal ribosomal operon, up to 6 Kb (18S-ITS1-5.8S-ITS2-28S) and to improve the taxonomy assignment of the communities up to species level and in real-time. We...

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Detalhes bibliográficos
Publicado no:Biol Methods Protoc
Main Authors: D’Andreano, Sara, Cuscó, Anna, Francino, Olga
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7820110/
https://ncbi.nlm.nih.gov/pubmed/33506108
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpaa026
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