NGS-PrimerPlex: High-throughput primer design for multiplex polymerase chain reactions

Multiplex polymerase chain reaction (PCR) has multiple applications in molecular biology, including developing new targeted next-generation sequencing (NGS) panels. We present NGS-PrimerPlex, an efficient and versatile command-line application that designs primers for different refined types of ampl...

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Bibliografiske detaljer
Udgivet i:PLoS Comput Biol
Main Authors: Kechin, Andrey, Borobova, Viktoria, Boyarskikh, Ulyana, Khrapov, Evgeniy, Subbotin, Sergey, Filipenko, Maxim
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2020
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7802936/
https://ncbi.nlm.nih.gov/pubmed/33378360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pcbi.1008468
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