Time-resolved cryoEM using Spotiton

We present an approach for preparing cryoEM grids to study short-lived molecular states. Using piezo electric dispensing, two independent streams of ~50 pL sample drops are deposited within 10 ms of each other onto a nanowire EM grid surface, and the mixing reaction stops when the grid is vitrified...

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Publicado en:Nat Methods
Main Authors: Dandey, Venkata P., Budell, William C., Wei, Hui, Bobe, Daija, Maruthi, Kashyap, Kopylov, Mykhailo, Eng, Edward T., Kahn, Peter A., Hinshaw, Jenny E., Kundu, Nidhi, Nimigean, Crina M., Fan, Chen, Sukomon, Nattakan, Darst, Seth A., Saecker, Ruth M., Chen, James, Malone, Brandon, Potter, Clinton S., Carragher, Bridget
Formato: Artigo
Idioma:Inglês
Publicado: 2020
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7799389/
https://ncbi.nlm.nih.gov/pubmed/32778833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-020-0925-6
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