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Refractive Index Imaging of Cells with Variable-Angle Near-Total Internal Reflection (TIR) Microscopy

The refractive index in the interior of single cells affects the evanescent field depth in quantitative studies using total internal reflection (TIR) fluorescence, but often that index is not well known. We here present method to measure and spatially map the absolute index of refraction in a micros...

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Publicat a:Microsc Microanal
Autors principals: Bohannon, Kevin P., Holz, Ronald W., Axelrod, Daniel
Format: Artigo
Idioma:Inglês
Publicat: 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7790292/
https://ncbi.nlm.nih.gov/pubmed/28918767
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1017/S1431927617012570
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