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Visualization of Regulated Exocytosis with a Granule-Membrane Probe Using Total Internal Reflection Microscopy

Secretory granules labeled with Vamp-green fluorescent protein (GFP) showed distinct signatures upon exocytosis when viewed by total internal reflection fluorescence microscopy. In ∼90% of fusion events, we observed a large increase in fluorescence intensity coupled with a transition from a small pu...

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Detalhes bibliográficos
Main Authors: Allersma, Miriam W., Wang, Li, Axelrod, Daniel, Holz, Ronald W.
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Cell Biology 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC519157/
https://ncbi.nlm.nih.gov/pubmed/15282339
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E04-02-0149
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