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Visualization of Regulated Exocytosis with a Granule-Membrane Probe Using Total Internal Reflection Microscopy
Secretory granules labeled with Vamp-green fluorescent protein (GFP) showed distinct signatures upon exocytosis when viewed by total internal reflection fluorescence microscopy. In ∼90% of fusion events, we observed a large increase in fluorescence intensity coupled with a transition from a small pu...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Cell Biology
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC519157/ https://ncbi.nlm.nih.gov/pubmed/15282339 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E04-02-0149 |
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