SpRY greatly expands the genome editing scope in rice with highly flexible PAM recognition

BACKGROUND: Plant genome engineering mediated by various CRISPR-based tools requires specific protospacer adjacent motifs (PAMs), such as the well-performed NGG, NG, and NNG, to initiate target recognition, which notably restricts the editable range of the plant genome. RESULTS: In this study, we th...

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Bibliografiske detaljer
Udgivet i:Genome Biol
Main Authors: Xu, Ziyan, Kuang, Yongjie, Ren, Bin, Yan, Daqi, Yan, Fang, Spetz, Carl, Sun, Wenxian, Wang, Guirong, Zhou, Xueping, Zhou, Huanbin
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2021
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7780387/
https://ncbi.nlm.nih.gov/pubmed/33397431
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-020-02231-9
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