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Structure of an affinity-matured inhibitory recombinant Fab against urokinase plasminogen activator reveals basis of potency and specificity

Affinity maturation of U33, a recombinant Fab inhibitor of uPA, was used to improve the affinity and the inhibitory effect compared to the parental Fab. Arginine scanning of the six CDR loops of U33 was done to identify initial binding determinants since uPA prefers arginine in its primary substrate...

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Bibliografski detalji
Izdano u:Biochim Biophys Acta Proteins Proteom
Glavni autori: Sevillano, N., Bohn, M.-F., Zimanyi, M, Chen, Y, Petzold, C, Gupta, S., Ralston, C.Y., Craik, C.S.
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7770097/
https://ncbi.nlm.nih.gov/pubmed/33221341
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2020.140562
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