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Purification and Use of tRNA for Enzymatic Post-translational Addition of Amino Acids to Proteins
Post-translational addition of amino acids to proteins by enzymes using aminoacyl-tRNA is an emerging regulatory mechanism. Examples include Arg transfer in eukaryotes, Leu/Phe transfer in bacteria, and tRNA-synthetase-mediated addition of amino acids to Lys side chains. Here, we present a method of...
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| Publicado no: | STAR Protoc |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7757669/ https://ncbi.nlm.nih.gov/pubmed/33377101 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100207 |
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