Isolation of Human CD49f(+) Astrocytes and In Vitro iPSC-Based Neurotoxicity Assays

Given the critical roles of astrocytes in neuroinflammation and neurological diseases, models for studying human astrocyte biology are in increasing demand. Here, we present a protocol to isolate human astrocytes from induced pluripotent stem cell (iPSC)-based cultures, neural organoids, and primary...

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Bibliografske podrobnosti
izdano v:STAR Protoc
Main Authors: Barbar, Lilianne, Rusielewicz, Tomasz, Zimmer, Matthew, Kalpana, Kriti, Fossati, Valentina
Format: Artigo
Jezik:Inglês
Izdano: Elsevier 2020
Teme:
Protocol
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757411/
https://ncbi.nlm.nih.gov/pubmed/33377066
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100172
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