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Secret3D Workflow for Secretome Analysis

Secretome analysis is crucial to unravel extracellular processes. However, secreted proteins are difficult to detect in mass-spectrometry-based analysis due to contamination of serum proteins deriving from cell culture media and to high glycosylation, which hampers tryptic digestion. Secret3D workfl...

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Enregistré dans:
Détails bibliographiques
Publié dans:STAR Protoc
Auteurs principaux: Matafora, Vittoria, Bachi, Angela
Format: Artigo
Langue:Inglês
Publié: Elsevier 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757356/
https://ncbi.nlm.nih.gov/pubmed/33377056
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100162
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