Protocol for High-Resolution Mapping of Splicing Products and Isoforms by RT-PCR Using Fluorescently Labeled Primers

We describe an RT-PCR protocol that allows high-resolution mapping of splicing products and isoforms using fluorescently labeled primers. Each species contains one fluorescent group allowing a direct comparison of the different isoforms despite size differences. A custom-size ladder enables the prec...

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Vydáno v:STAR Protoc
Hlavní autoři: Weathers, Indya, Gabunilas, Jason, Samson, Joyce, Roy, Kevin, Chanfreau, Guillaume F.
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2020
Témata:
Protocol
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7757285/
https://ncbi.nlm.nih.gov/pubmed/33377034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100140
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