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PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data

The vast majority of multi-exon genes in higher eukaryotes are alternatively spliced and changes in alternative splicing (AS) can impact gene function or cause disease. High-throughput RNA sequencing (RNA-seq) has become a powerful technology for transcriptome-wide analysis of AS, but RT-PCR still r...

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Vydáno v:Genomics Proteomics Bioinformatics
Hlavní autoři: Tokheim, Collin, Park, Juw Won, Xing, Yi
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4411361/
https://ncbi.nlm.nih.gov/pubmed/24747190
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.gpb.2014.04.001
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