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Engineering of Thermostable β‐Hydroxyacid Dehydrogenase for the Asymmetric Reduction of Imines
The β‐hydroxyacid dehydrogenase from Thermocrinus albus (Ta‐βHAD), which catalyzes the NADP(+)‐dependent oxidation of β‐hydroxyacids, was engineered to accept imines as substrates. The catalytic activity of the proton‐donor variant K189D was further increased by the introduction of two nonpolar flan...
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| Publicado no: | Chembiochem |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
John Wiley and Sons Inc.
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7756219/ https://ncbi.nlm.nih.gov/pubmed/32939899 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.202000526 |
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