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Differential detergent fractionation of membrane protein from small samples of hepatocytes and liver tissue for quantitative proteomic analysis of drug metabolizing enzymes and transporters

The fractionation of enough membrane protein from limited samples is challenging for MS-based quantitative targeted absolute proteomics (QTAP) of drug metabolizing enzymes (DMEs) and transporters. This study evaluated differential detergent fractionation (DDF) of membrane protein from progressively...

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Bibliografiske detaljer
Udgivet i:J Pharm Sci
Main Authors: Qasem, Rani J., Fallon, John K., Nautiyal, Manisha, Mosedale, Merrie, Smith, Philip C.
Format: Artigo
Sprog:Inglês
Udgivet: 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7750260/
https://ncbi.nlm.nih.gov/pubmed/33148403
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xphs.2020.10.037
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