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A simple method using CRISPR-Cas9 to knock-out genes in murine cancerous cell lines

CRISPR-Cas9 system can be used to generate knock-out cancer cell lines. An insertion or deletion induced by a single guide RNA (gRNA) is often used to generate knock-out cells, however, some cells express the target gene by skipping the disrupted exon, or by using a splicing variant, thus losing the...

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Podrobná bibliografie
Vydáno v:Sci Rep
Hlavní autoři: Ishibashi, Airi, Saga, Kotaro, Hisatomi, Yuuta, Li, Yue, Kaneda, Yasufumi, Nimura, Keisuke
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7749119/
https://ncbi.nlm.nih.gov/pubmed/33339985
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-79303-0
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