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A method for conditional regulation of protein stability in native or near-native form

Here, we report a method to regulate cellular protein levels by introducing a ubiquitin variant between a destabilizing domain (DD) and the regulated protein. When produced in the absence of a stabilizing ligand the DD dominates and the entire fusion protein is processively degraded by the proteasom...

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Detalhes bibliográficos
Publicado no:Cell Chem Biol
Main Authors: Miyamae, Yusaku, Chen, Ling-chun, Utsugi, Yuki, Farrants, Helen, Wandless, Thomas J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7749034/
https://ncbi.nlm.nih.gov/pubmed/33007216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2020.09.004
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