A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila

Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out. However, technical challenges remain to improve efficiency and broaden applicability to any genetic background...

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Bibliografske podrobnosti
izdano v:Fly (Austin)
Main Authors: Nyberg, Kevin G., Nguyen, Joseph Q., Kwon, Yong-Jae, Blythe, Shelby, Beitel, Greg J., Carthew, Richard
Format: Artigo
Jezik:Inglês
Izdano: Taylor & Francis 2020
Teme:
Methods and Technical Advances
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7746241/
https://ncbi.nlm.nih.gov/pubmed/33016195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/19336934.2020.1832416
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