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A pipeline for precise and efficient genome editing by sgRNA-Cas9 RNPs in Drosophila

Genome editing via homology-directed repair (HDR) has made possible precise and deliberate modifications to gene sequences. CRISPR/Cas9-mediated HDR is the simplest means to carry this out. However, technical challenges remain to improve efficiency and broaden applicability to any genetic background...

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Detalhes bibliográficos
Publicado no:Fly (Austin)
Main Authors: Nyberg, Kevin G., Nguyen, Joseph Q., Kwon, Yong-Jae, Blythe, Shelby, Beitel, Greg J., Carthew, Richard
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7746241/
https://ncbi.nlm.nih.gov/pubmed/33016195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/19336934.2020.1832416
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