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Mapping transmembrane binding partners for E-cadherin ectodomains
We combine proximity labeling and single molecule binding assays to discover transmembrane protein interactions in cells. We first screen for candidate binding partners by tagging the extracellular and cytoplasmic regions of a “bait” protein with BioID biotin ligase and identify proximal proteins th...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
2020
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7733791/ https://ncbi.nlm.nih.gov/pubmed/33229577 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2010209117 |
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