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Mapping transmembrane binding partners for E-cadherin ectodomains
We combine proximity labeling and single molecule binding assays to discover transmembrane protein interactions in cells. We first screen for candidate binding partners by tagging the extracellular and cytoplasmic regions of a “bait” protein with BioID biotin ligase and identify proximal proteins th...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7733791/ https://ncbi.nlm.nih.gov/pubmed/33229577 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2010209117 |
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