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Radical SAM Enzyme Spore Photoproduct Lyase: Properties of the Ω Organometallic Intermediate and Identification of Stable Protein Radicals Formed during Substrate-Free Turnover

Spore photoproduct lyase is a radical S-adenosyl-l-methionine (SAM) enzyme with the unusual property that addition of SAM to the [4Fe-4S](1+) enzyme absent substrate results in rapid electron transfer to SAM with accompanying homolytic S─C5′ bond cleavage. Herein, we demonstrate that this unusual re...

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Detalhes bibliográficos
Publicado no:J Am Chem Soc
Main Authors: Pagnier, Adrien, Yang, Hao, Jodts, Richard J., James, Christopher D., Shepard, Eric M., Impano, Stella, Broderick, William E., Hoffman, Brian M., Broderick, Joan B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7730139/
https://ncbi.nlm.nih.gov/pubmed/32966073
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.0c08585
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