Dual Fluorescence Splicing Reporter Minigene Identifies an Antisense Oligonucleotide to Skip Exon v8 of the CD44 Gene

Splicing reporter minigenes are used in cell-based in vitro splicing studies. Exon skippable antisense oligonucleotide (ASO) has been identified using minigene splicing assays, but these assays include a time- and cost-consuming step of reverse transcription PCR amplification. To make in vitro splic...

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Veröffentlicht in:Int J Mol Sci
Hauptverfasser: Fukushima, Sachiyo, Farea, Manal, Maeta, Kazuhiro, Rani, Abdul Qawee Mahyoob, Fujioka, Kazumichi, Nishio, Hisahide, Matsuo, Masafumi
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2020
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7729581/
https://ncbi.nlm.nih.gov/pubmed/33266296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21239136
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