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Detection and Quantification of RNA Phosphorothioate Modification Using Mass Spectrometry
This unit describes the protocol to detect and quantify RNA phosphorothioate (PS) modifications in cellular RNA samples. Starting from the solid-phase synthesis of phosphorothioate RNA dinucleotides, followed by purification with reverse-phase HPLC, we prepared phosphorothioate RNA dinucleotide stan...
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| Publicado no: | Curr Protoc Nucleic Acid Chem |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7700719/ https://ncbi.nlm.nih.gov/pubmed/32822120 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpnc.113 |
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