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Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA

The supercoiled circular (SC) topology form of plasmid DNA has been regarded to be advantageous over open circular or linearized analogue in transfection and expression efficiency, and therefore are largely demanded in the biopharmaceutical manufacturing. However, production of high-purity SC plasmi...

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Podrobná bibliografie
Vydáno v:Cytotechnology
Hlavní autoři: Lu, Xin-An, He, Ting, Han, Zhihai, Ding, Yanping, Zhao, Liang, Liu, Guanghua, De Smet, Floris, Huang, Xiaojun, Chen, Danqing, Qi, Feifei, Zhao, Xiangyu
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer Netherlands 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7695760/
https://ncbi.nlm.nih.gov/pubmed/33123933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-020-00433-4
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