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Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

Fast photochemical oxidation of proteins (FPOP) is a mass spectrometry-based structural biology technique that probes the solvent-accessible surface area of proteins. This technique relies on the reaction of amino acid side chains with hydroxyl radicals freely diffusing in solution. FPOP generates t...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Misra, Sandeep K., Sharp, Joshua S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7695538/
https://ncbi.nlm.nih.gov/pubmed/32955502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/61580
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