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Extraction of recombinant periplasmic proteins under industrially relevant process conditions: Selectivity and yield strongly depend on protein titer and methodology

In this work, we attempted to identify a method for the selective extraction of periplasmic endogenously expressed proteins, which is applicable at an industrial scale. For this purpose, we used an expression model that allows coexpression of two fluorescent proteins, each of which is specifically t...

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Detalhes bibliográficos
Publicado no:Biotechnol Prog
Main Authors: Schimek, Clemens, Egger, Esther, Tauer, Christopher, Striedner, Gerald, Brocard, Cécile, Cserjan‐Puschmann, Monika, Hahn, Rainer
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley & Sons, Inc. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7685146/
https://ncbi.nlm.nih.gov/pubmed/32259401
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/btpr.2999
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