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Fast and antibiotic free genome integration into Escherichia coli chromosome
Genome-based Escherichia coli expression systems are superior to conventional plasmid-based systems as the metabolic load triggered by recombinant compounds is significantly reduced. The efficiency of T7-based transcription compensates for low gene dosage (single copy) and facilitates high product f...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7536200/ https://ncbi.nlm.nih.gov/pubmed/33020519 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-73348-x |
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