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Kinetic characterization and structure analysis of an altered polyol dehydrogenase with d‐lactate dehydrogenase activity
During adaptive metabolic evolution a native glycerol dehydrogenase (GDH) acquired a d‐lactate dehydrogenase (LDH) activity. Two active‐site amino acid changes were detected in the altered protein. Biochemical studies along with comparative structure analysis using an X‐ray crystallographic structur...
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| Udgivet i: | Protein Sci |
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| Main Authors: | , , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
John Wiley & Sons, Inc.
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7679959/ https://ncbi.nlm.nih.gov/pubmed/33020946 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3963 |
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