Kinetic characterization and structure analysis of an altered polyol dehydrogenase with d‐lactate dehydrogenase activity

During adaptive metabolic evolution a native glycerol dehydrogenase (GDH) acquired a d‐lactate dehydrogenase (LDH) activity. Two active‐site amino acid changes were detected in the altered protein. Biochemical studies along with comparative structure analysis using an X‐ray crystallographic structur...

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Bibliographic Details
Published in:Protein Sci
Main Authors: Chauliac, Diane, Wang, Qingzhao, St. John, Franz J., Jones, Grace, Hurlbert, Jason C., Ingram, Lonnie O., Shanmugam, Keelnatham T.
Format: Artigo
Language:Inglês
Published: John Wiley & Sons, Inc. 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7679959/
https://ncbi.nlm.nih.gov/pubmed/33020946
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3963
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