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Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals

Measurement of cytokine gene expression by reverse transcription quantitative polymerase chain reaction (RT-qPCR) is used widely to assess the immune system of animals and to identify biomarkers of disease, but its application is limited in wildlife species due to a lack of species-specific reagents...

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Detalhes bibliográficos
Publicado no:PeerJ
Main Authors: Meza Cerda, María-Ignacia, Gray, Rachael, Higgins, Damien P.
Formato: Artigo
Idioma:Inglês
Publicado em: PeerJ Inc. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7668205/
https://ncbi.nlm.nih.gov/pubmed/33240637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.10306
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