Upstream development of Escherichia coli fermentation process with PhoA promoter using design of experiments (DoE)

In this work, a fed-batch fermentation development was performed with recombinant E. coli carrying the PhoA promoter system. The phosphate concentrations tested for this PhoA strain, 2.79 mM to 86.4 mM, were beyond the concentrations previously evaluated for cell growth and product titer. The result...

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Bibliografiske detaljer
Udgivet i:J Ind Microbiol Biotechnol
Main Authors: Agbogbo, Frank K., Ramsey, Phil, George, Renija, Joy, Jobin, Srivastava, Shikha, Huang, Mian, McCool, Jesse
Format: Artigo
Sprog:Inglês
Udgivet: Springer International Publishing 2020
Fag:
Fermentation, Cell Culture and Bioengineering - Original Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7658055/
https://ncbi.nlm.nih.gov/pubmed/32844325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10295-020-02302-7
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