Efficient recovery of the RNA-bound proteome and protein-bound transcriptome using phase separation (OOPS)

RNA-protein interactions play a pivotal role in cell homeostasis and disease, but current approaches to study them require a considerable amount of starting material, favor the recovery of only a subset of RNA species or are complex and time-consuming. We recently developed orthogonal organic phase...

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Publicat a:Nat Protoc
Autors principals: Villanueva, Eneko, Smith, Tom, Queiroz, Rayner M. L., Monti, Mie, Pizzinga, Mariavittoria, Elzek, Mohamed, Dezi, Veronica, Harvey, Robert F., Ramakrishna, Manasa, Willis, Anne E., Lilley, Kathryn S.
Format: Artigo
Idioma:Inglês
Publicat: 2020
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7613161/
https://ncbi.nlm.nih.gov/pubmed/32651564
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-0344-2
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