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Quantification of mRNA cap-modifications by means of LC-QqQ-MS
Enzymatic modification of the 5′-cap is a versatile approach to modulate the properties of mRNAs. Transfer of methyl groups from S-adenosyl-l-methionine (AdoMet) or functional moieties from non-natural analogs by methyltransferases (MTases) allows for site-specific modifications at the cap. These mo...
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| Published in: | Methods |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Academic Press
2022
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7612805/ https://ncbi.nlm.nih.gov/pubmed/34058305 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2021.05.018 |
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