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Quantification of mRNA cap-modifications by means of LC-QqQ-MS

Enzymatic modification of the 5′-cap is a versatile approach to modulate the properties of mRNAs. Transfer of methyl groups from S-adenosyl-l-methionine (AdoMet) or functional moieties from non-natural analogs by methyltransferases (MTases) allows for site-specific modifications at the cap. These mo...

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Bibliografske podrobnosti
izdano v:Methods
Main Authors: Muthmann, Nils, Špaček, Petr, Reichert, Dennis, van Dülmen, Melissa, Rentmeister, Andrea
Format: Artigo
Jezik:Inglês
Izdano: Academic Press 2022
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7612805/
https://ncbi.nlm.nih.gov/pubmed/34058305
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2021.05.018
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