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Responsive fluorescent nucleotides serve as efficient substrates to probe terminal uridylyl transferase†
We repurposed a terminal uridylyl transferase enzyme to site-specifically label RNA with microenvironment sensing fluorescent nucleotide mimics, which in turn provided direct read-outs to estimate the binding affinities of the enzyme to RNA and nucleotide substrates. This enzyme–probe system provide...
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| Udgivet i: | Chem Commun (Camb) |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7611084/ https://ncbi.nlm.nih.gov/pubmed/32939524 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/d0cc05092j |
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