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Integrative expression vectors with Pgrac promoters for inducer-free overproduction of recombinant proteins in Bacillus subtilis

Inducer-free integrative vectors are often used to create B. subtilis strains for industrial purposes, but employing strong promoters to produce high levels of recombinant proteins in B. subtilis results in high leaky expression that can hamper cloning in Escherichia coli. To overcome the problem, w...

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Vydáno v:Biotechnol Rep (Amst)
Hlavní autoři: Tran, Dinh Thi Minh, Phan, Trang Thi Phuong, Doan, Thanh Thi Ngoc, Tran, Thuoc Linh, Schumann, Wolfgang, Nguyen, Hoang Duc
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2020
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7599426/
https://ncbi.nlm.nih.gov/pubmed/33163371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2020.e00540
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