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Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli
BACKGROUND: In general, fusion of recombinant genes to strong inducible promoters allowing intracellular expression in Bacillus subtilis is a two-step process. The ligation products are transformed into Escherichia coli, followed by identification of the correct plasmid, and this plasmid is subseque...
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| Publicado en: | Microb Cell Fact |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BioMed Central
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4446860/ https://ncbi.nlm.nih.gov/pubmed/25990516 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0255-z |
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