Combined Approach to Engineer a Highly Active Mutant of Processive Chitinase Hydrolyzing Crystalline Chitin

[Image: see text] Serratia marcescens chitinase A (SmChiA) processively hydrolyzes recalcitrant biomass crystalline chitin under mild conditions. Here, we combined multiple sequence alignment, site-saturation mutagenesis, and automated protein purification and activity measurement with liquid-handli...

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Vydáno v:ACS Omega
Hlavní autoři: Visootsat, Akasit, Nakamura, Akihiko, Wang, Tak-Wai, Iino, Ryota
Médium: Artigo
Jazyk:Inglês
Vydáno: American Chemical Society 2020
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7581260/
https://ncbi.nlm.nih.gov/pubmed/33111007
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acsomega.0c03911
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