A Biologist’s Guide to Traction Force Microscopy Using Polydimethylsiloxane Substrate for Two-Dimensional Cell Cultures

Cellular traction forces influence epithelial behavior, including wound healing and cell extrusion. Here, we describe a simple in vitro traction force microscopy (TFM) protocol using ECM protein-coated polydimethylsiloxane substrate and widefield fluorescence microscopy. We include detailed steps fo...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:STAR Protoc
Prif Awduron: Teo, Jessica L., Lim, Chwee Teck, Yap, Alpha S., Saw, Thuan Beng
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Elsevier 2020
Pynciau:
Protocol
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580222/
https://ncbi.nlm.nih.gov/pubmed/33111126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100098
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